Coding

Part:BBa_K4429009:Design

Designed by: Anish Mulchandani   Group: iGEM22_IISER-Pune2_India   (2022-07-28)


NeoFv C10 VH-LinY12H-VL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

FcRn binding peptide variant generated according to Sockolosky et al 2012 [1], attached at the right location on the scFv BBa_K4429008 using SnapGene. Protein sequence reverse translated and optimised for expression in E. coli K12.


Source

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References

[1] Sockolosky, J. T., Tiffany, M. R., & Szoka, F. C. (2012). Engineering neonatal Fc receptor-mediated recycling and transcytosis in recombinant proteins by short terminal peptide extensions. Proceedings of the National Academy of Sciences of the United States of America, 109(40), 16095–16100. https://doi.org/10.1073/pnas.1208857109